METHOD OF PRODUCING ROOTED CUTTINGS OF TREE BELONGING TO THE FAMILY OF PINACEAE

摘要

The present invention provides a method for producing a rooted cuttingwhich can be applied to Pinaceae trees. Specifically, the present inventionprovides amethod for producing a rooted cutting of a Pinaceae tree, comprising thefollowingsteps A and B.A: adding a cytokinin to the outside of a shoot apex of a short shoot of aPinaceae tree to thereby induce a new scaly leaf and/or primary leaf;B: cutting out the short shoot where new scaly leaf and/or primary leaf isinduced in the step A, inserting the short shoot as a cutting in a rooting bedwetted witha carbon source-free liquid medium comprising nitrogen, phosphorus andpotassium asessential elements, and culturing the short shoot in a culturing vessel whileregulatingthe concentration of CO2 gas in the culturing vessel at a humidity of 80% ormore tothereby root the cutting.