Broad-spectrum antibacterial and antifungal activity of Lactobacillus johnsonii D115

摘要

The present invention demonstrated the potential use of Lactobacillus johnsoniiD115 as a probiotic, as a prophylactic agent or as a surface treatment of materialsagainst human and animal pathogens such as Brachyspira pilosicoli, Brachyspirahyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus,Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis,Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica,Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonellaspp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. Theproteineous antimicrobial compound was partially characterized and foundto be heat tolerant up to 121°C for 15 min, and acid tolerant up to pH1 for 30min at 40°C. The compound is also stable to enzymatic digestion, being ableto retain more than 60% antimicrobial activity when treated with pepsin and trypsin.