Improved transfection of eukaryotic cells with linear polynucleotides by electroporation

摘要

#$%^&*AU2011201652C120130314.pdf#####C:NRPortblDCCKXG L741022 1 DOC-841201 I ABSTRACT The present invention provides an improved method for gene delivery in eukaryotic cells 5 by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.