REHYBRIDIZATION PROBE SYSTEM FOR THE QUALITATIVE AND QUANTITATIVE MEASUREMENT OF SPECIFIC NUCLEIC ACIDS IN REAL-TIME

摘要

The method for the real-time or end-point detection of nucleic acids is carried out using rehybridization probe systems. The novel probe system is characterized in that the system allows a homogenous and high-throughput nucleic acid detection that can be automated. Using the rehybridization probe system, a multiplex detection is also possible. In a particularly efficient embodiment of the invention, two probes are used for a qualitative or quantitative real-time measurement of specific target nucleic acids. In this manner, the detection sensitivity and signal strength can be substantially increased, and a target differentiation can be achieved.