METHOD FOR IMMOBILIZING PROTEIN ON SELF-ASSEMBLED FILM

摘要

A purpose of the present invention is to increase the amount of protein immobilized on a self-assembled film in order to improve the detection sensitivity or the quantitative precision of a target substance. The present invention is characterized in that a single molecule of an amino acid selected from five types of amino acids comprising cysteine, lysine, histidine, phenylalanine, and glycine is inserted between a self-assembled film and a protein molecule. The present invention provides, for example, a method for immobilizing a protein on a self-assembled film, wherein the method is provided with steps (a) and (b) in the stated order: (a) preparing a substrate provided with a single molecule of an amino acid and a self-assembled film; and (b) supplying a protein onto the substrate, and forming a peptide bond expressed by a predetermined chemical formula as a result of a reaction between a carboxyl group of the single molecule of amino acid and an amino group of the protein.