LIVE, ORAL VACCINE FOR PROTECTION AGAINST SHIGELLA DYSENTERIAE SEROTYPE 1

摘要

The present invention relates to Salmonella typhi Ty21a a comprising core-linked Shigella dysenteriae type 1 O-specific polysaccharide (O-Ps) and DNA encoding O antigen biosynthesis, said DNA selected from the group consisting of: a) the DNA sequence set out in any one of SEQ ID NOs: 1 and 2; b) DNA encoding S. dysenteriae serotype 1 biosynthesis polypeptides encoded by any one of SEQ ID NOs: 1 and 2; c) DNA encoding variants of the polypeptide encoded by the DNA of (a) or (b), wherein the variants comprise O antigen biosynthesis polypeptide analogs, wherein one or more of the specified amino acids is deleted or replaced, or wherein one or more non-specified amino acids are added without loss of biosynthesis of Shigella dysenteriae serotype 1 O antigen or protective immunological activity of the O antigen biosynthesis gene product; and d) nucleic acid molecules encoding gene products that share at least about 90% sequence identity with the polypeptide encoded by the nucleic acid molecule of SEQ ID NO: 1 or 2.