METHOD FOR THE DETECTION OF MULTIPLE SINGLE NUCLEOTIDE VARIATIONS OR SINGLE NUCLEOTIDE POLYMORPHISMS IN A SINGLE TUBE

摘要

The present invention discloses a method for detecting multiple single nucleotide variations or polymorphisms in a single reaction tube, and the oligonucleotide, the probe, the set of probes, the kit used, as well as the use thereof. Specifically, it relates to a method for identifying the genotype of multiple single nucleotide variation or SNP sites from the melting temperature of a kind of artificial melting temperature tag sequence (AMTS ) and the type of fluorescence labels.