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XYLITOL-PRODUCING STRAIN TO WHICH AN ARABINOSE METABOLIC PATHWAY IS INTRODUCED, AND METHOD FOR PRODUCING XYLITOL USING SAME
XYLITOL-PRODUCING STRAIN TO WHICH AN ARABINOSE METABOLIC PATHWAY IS INTRODUCED, AND METHOD FOR PRODUCING XYLITOL USING SAME
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机译:引入产生木糖醇的菌株,其引入了阿拉伯糖代谢途径,以及使用该方法生产木糖醇的方法
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摘要
The present invention relates to an efficient production method of xylitol by using the xylitol producing microorganism introduced with arabinose metabolic pathway to inhibit the production of arabitol, the byproduct, and instead to use arabinose only for cell metabolism in xylose/arabinose mixed medium. More precisely, to express efficiently L-arabinose isomerase (araA), L-ribulokinase (araB) and L-ribulose-5-phosphate 4-epimerase (araD) in Candida tropicalis, codon optimization was performed. Then, each gene was inserted in the gene expression cassette containing the glyceraldehyde-3-phosphate dehydrogenase promoter and the selection marker URA3, which was introduced into Candida sp. microorganism. As a result, arabitol, the byproduct interrupting the purification and crystallization of xylitol could be inhibited, making the production method of xylitol of the present invention more efficient. The xylitol producing microorganism introduced with arabinose metabolic pathway of the present invention can be effectively used for the production of xylitol with high productivity by inhibiting the generation of arabitol.
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