METHOD OF WASHING CELLS USING PASSIVE SEPARATION OF A LYSED BLOOD SAMPLE WITH CLEAN BUFFER USING INERTIAL MICROFLUIDIC SEPARATION AND FOCUSING IN SPIRAL MICROCHANNELS

摘要

A method and apparatus for the automatic, manual-free washing of stained, lysed cells as an anterior step to delivering the washed cell stream to a flow cytometer for subsequent counting and analysis is disclosed, which utilizes a passive, automatic, self-separating, inertial microfluidic flow in microchannels, whereby the washed cells are passively separated from a lysed solution and focused to the center microchannel of the device.