PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS

摘要

Overexpression coding glucose-6-phosphate dehydrogenase (G6PD) [" G6PDH "] and [6-phospho-gluconolactonase " 6PGL "] of gene synergic adjustment is in the saccharomyces olei Yarrowialipolytica of transgenic strain, including functional polyunsaturated fatty acid [" PUFA "] biosynthesis pathway, causes to produce PUFAs and increase the yarrowia cell of total lipid content. This is by the reduced nicotinamide adenine dinucleotides phosphoric acid [" NADPH "] of increase honeycomb availability, and important reducing equivalent is used for reproducibility biosynthesis reaction, in transgenic microorganism.