METHOD AND KIT FOR DETECTING MACROLIDE ANTIBIOTIC-RESISTANT MUTANT BACTERIUM

摘要

Provided is a method for detecting a macrolide antibiotic-resistant mutant bacterium rapidly and accurately. A macrolide antibiotic-resistant mutant bacterium is detected by a real-time PCR using a first hydrolysis probe and a second hydrolysis probe. A part of the sequence for the first hydrolysis probe is composed of LNA. In the second hydrolysis probe, the 5'-termial is labeled with a reporter fluorescent dye and the 3'-terminal is labeled with a quencher non-fluorescent dye and a minor groove binder (MGB). When a macrolide antibiotic-sensitive bacterium is detected, both fluorescence coming from the first hydrolysis probe and fluorescence coming from the second hydrolysis probe are observed. When a macrolide antibiotic-resistant mutant bacterium is detected, only fluorescence coming from the second hydrolysis probe is observed.