APPLICATION OF LIGHT SCATTERING PATTERNS TO DETERMINE DIFFERENTIATION STATUS OF STEM CELLS AND STEM CELL COLONIES

摘要

A method for identifying viability and/or differentiation status of cell colonies by analysis of scattergrams of colonies is disclosed. Scattergrams are obtained by cultering samples and illuminating the resultant colonies by a laser. The forward scattered light is imaged and subject to a feature extraction process. The feature vector may include Zernike or Chebyshev moments and may also include Harelick texture features. Feature vectors may be used to train a classification process using either supervised or unsupervised machine learning techniques. The classification process may be used to associate a colony phenotype with the genotype of the sample.