Method for generating primate cardiovascular progenitor cells for clinical and drug cells testing use from primate embryonic stem cells or embryonic-like state cells, and their applications

摘要

The present invention is directed to a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from human, wherein said method comprises the use of reconstituted basement membrane-derived extracellular composition (named "matrigel"), particularly from EHS (Engelbreth Holm-Swarm) tumor extract and the SSEA1 marker as a positive cardiovascular progenitors differentiation marker with BMP-2. Derived cell culture medium compositions and kit in relation with the method of the invention or product obtainable by said method form also part of the present invention. The present invention is further directed to method for testing agents for effect on human cardiovascular cells.