A Method for the Toxicity Assessments of Nano-Materials using Selective Plane Illumination Microcopy

摘要

PURPOSE: A method for evaluating nano-material risk is provided to obtain accurate and reproducible result. CONSTITUTION: A method for evaluating nano-material risk in real-time comprises: a step of exposing the nano-material to a cell medium; a step of measuring the number and size of the nano-material dispersed in the cell medium using two or more SPIM(selectively plane illumination microscopy); and a step of analyzing the effect for cells of the nano-material using the result. The SPIM is formed of a laser-based fluorescent microscope of a dark field. The number of the nano-materials dispersed in the cell medium is measure through a nano-material image analysis.