SOLID SUPPORT FOR PURIFYING HISTIDINE-TAGGED PROTEIN, WHICH IS CONJUGATED WITH A HIS-TAG APTAMER THROUGH DOUBLE SULFUR BOND AND A METHOD FOR PURIFYING THE SAME

摘要

PURPOSE: A solid support for purifying histidine-tagged protein is provided to ensure superior safety over Ni^2+-based affinity chromatography purification and to reduce cost.;CONSTITUTION: A solid support with histidine-tagged protein is prepared by double sulfur bond of a His-tag aptamer to mercaptomethyl polystyrene resin. The His-tag aptamer is an RNA aptamer with a base sequence of sequence number 2. The His-tag is an oligohistidine tag. A method for purifying the histidine-tagged protein comprises a step of selective interaction between the histidine-tagged aptamer and oligohistidine.;COPYRIGHT KIPO 2013