TWO-PHOTON LYSOTRACKERS FOR IN VIVO IMAGING, METHOD FOR PREPARING THE SAME AND METHOD FOR IN VIVO IMAGING OF LYSOSOMES

摘要

PURPOSE: A two-photon lysosome marker for in vivo imaging is provided to detect lysosomes in cells and tissues by a two-phton microscopy by easily loading into the cells and tissues. CONSTITUTION: A two-photon lysosome marker for in vivo imaging is denoted by chemical formula 1. In chemical formula 1, X is -OCH_3 or -N(CH_3)_2, wherein the marker has a maximum value of luminescence at 470 nm when X is -OCH_3, and the marker has a maximum value of luminescence at 550 nm when X is -N(CH_3)_2. A method for preparing the marker comprises a step of reacting a compound of chemical formula 2 with N,N-dimethylethylene diamine. A method for in vivo imaging of lysosome comprises: a step of injecting the compound of chemical formula 1 into an organ; and a step of detecting two-photon fluorescence.