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COMPOSITE OF MAGNETIC NANOPARTICLES AND ADENO-ASSOCIATED VIRUS, WHICH IMPROVES GENE DELIVERY SPEED AND EFFICIENCY IN A NON-INVASIVE CELL, AND A GENE DELIVERY METHOD INTO A CELL USING THE SAME
COMPOSITE OF MAGNETIC NANOPARTICLES AND ADENO-ASSOCIATED VIRUS, WHICH IMPROVES GENE DELIVERY SPEED AND EFFICIENCY IN A NON-INVASIVE CELL, AND A GENE DELIVERY METHOD INTO A CELL USING THE SAME
PURPOSE: A composite of magnetic nanoparticles and adeno-associated virus, and a gene delivery method into a cell using the same are provided to deliver a foreign gene into a non-invasive cell type, to control moving speed of a magnet, and to control foreign gene delivery speed.;CONSTITUTION: A composite contains superparamagnetic nanoparticles which are conjugated through a streptavidin-biotin-NTA(nitrilotriacetic acid)-nickel-hexa histidine linker, and an adeno-associated virus(AAV) vector. The superparamagnetic nanoparticles are gamma-Fe2O3, Fe3O4, or a mixture thereof. The adeno-associated virus vector is AAV2 vector containing cytomegalovirus(CMV) promoter and hexa histidine at a 587th site of LQRGNLGHHHHHHSRQA-(corresponding to wild type AAV2:^583 LQRGNRQA). A gene delivery method into a cell comprises: a step of delivering a gene delivery system into the non-invasive cell type using the magnet; and a step of controlling moving speed of the magnet for controlling gene delivery speed.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Streptavidin; (BB) Biotin; (CC) Hexa histidine
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机译:目的:提供磁性纳米粒子和腺相关病毒的复合物,以及使用该复合物的基因传递方法,将外源基因传递至非侵入性细胞类型,以控制磁体的移动速度,并控制组成:复合物包含通过顺链亲和素-生物素-NTA(亚硝酸三乙酸)-镍-六组氨酸连接体和腺相关病毒(AAV)载体缀合的超顺磁性纳米颗粒。超顺磁性纳米颗粒是γ-Fe2 O 3,Fe 3 O 4或它们的混合物。腺相关病毒载体是在LQRGNLGHHHHHHSRQA-的第587位(对应于野生型AAV2:^ 583 LQRGNRQA)包含巨细胞病毒(CMV)启动子和六组氨酸的AAV2载体。一种向细胞内传递基因的方法,包括以下步骤:使用磁铁向非侵入性细胞类型内传递基因。 COPYRIGHT KIPO 2013; [参考数字](AA)链霉亲和素;以及控制磁体传递速度的步骤。 (BB)生物素; (CC)六聚组氨酸
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