MUD FLAT METAGENOME-DERIVED NOVEL GENE AND A NOVEL PROTEIN WITH PHOSPHOLIPASE AND LIPASE ACTIVITIES, PREPARED FROM THE SAME

摘要

PURPOSE: A novel gene isolated from mud flat metagenome library and a protein encoded by the same are provided to enable mass production and to lower temperature activity and stability.;CONSTITUTION: A polypeptide with phospholipase and lipase activities has an amino acid sequence of sequence number 5. Phospholipase and lipase are active at Ph 5-10 and at 3-30 deg. C. A polynucleotide encodes the polypeptide. A recombinant expression vector contains the polynucleotide. A transformant(deposit number KCTC 11942BP) is prepared by introducing the recombinant expression vector into a host cell.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Activity in a solid medium in which 1% tricaplyrin is added; (BB) Activity in a solid medium in which 1% phosphatidylcholine is added