SETS OF PRIMERS AND TAQMAN MGB PROBES FOR REAL-TIME PCR-BASED ASSAYS TO DISCRIMINATE GINSENG CULTIVARS

摘要

PURPOSE: A primer for the ginseng fingerprinting and probe set is quick with the realtime PCR reaction only DNA of the specific base sequence region. It amplifies. And the amplification amount can be quantitively measured. CONSTITUTION: A gene expression analysis set for the ginseng fingerprinting comprises the probe noted in the base sequence of the primer set consisting of the base sequence of the sequence number 5 and sequence number 6 and sequence number 7 to the beak (VIC) and the probe which it is hollowed in the base sequence of the sequence number 8 it notes to (FAM). The ginseng species is the cloth Chunpoong, breeze Yunpoong, antiquity Gopoong, autumnal breeze Kumpoong or the cyclonic Sunpoong. The base sequence of the probe and primer set originates from the ginseng (Panax ginseng C. A. Meyer). The method for quantitively measuring with the realtime PCR analysis method comprises the following steps. The ginseng species gene is quantitively measured with the realtime PCR analysis method. The realtime PCR analysis may use the gene expression analysis set.