RAPID ANTIBIOTIC SUSCEPTIBILITY TEST (RAST) SYSTEM BASED ON BACTERIAL FIXATION, ANTIBIOTIC DIFFUSION AND SINGLE CELL GROWTH TRACKING USING GELLING AGENTS

摘要

PURPOSE: A RAST system is provided to sharply reduce an amount of cells and drugs which are necessary for a doping test and obtain a doping test result within 2 hours by rapidly tracking the difference in growth of a single cell.;CONSTITUTION: A biological activity test structure is equipped with a solid film which is manufactured by gelling liquid medium containing hardening agents. The biological activity test structure comprises: a light-transmissive body portion; at least one micro well which is formed in the light-transmissive body portion; and the thin solid film which is equipped in the micro well and fixes biological material. The biological activity test structure is combinable with multi-well plates. At least one micro well of the light-transmissive body portion is corresponded to at least one well of the multi-well plates when the biological activity test structure is bonded with the multi-well plates.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Start; (BB) End; (S1) Supplying a mixture solution of liquid medium and biological materials to a thin film formation substrate; (S2) Forming a solid thin film to which biological materials are fixed by solidifying the mixture solution; (S3) Permeating physiologically active materials into a solid thin film; (S4) Observing biological materials against physiologically active materials