COLORIMETRIC FOCUS-FORMING ASSAY FOR QUANTIFICATION OF NONCYTOPATHIC HEPATITIS VIRUS AND AUTOMATED FOCUS COUNTING BY IMAGE ANALYSIS?‹

摘要

?‹The present invention relates to a method for more efficiently and objectively quantifying noncytopathic hepatitis virus, and more particularly, to a colorimetric focus-forming assay characterized by using BICP/NBT chromogen with which alkaline-phosphatase enzyme is combined, and an automated focus counting method using an automatic image analysis function. The colorimetric focus-forming assay and the automated focus counting method by image analysis according to the present invention are objective by excluding an inspector's judgement in comparison with the existing immunofluorescence-based focus-forming assay and useful to effectively quantify noncytopathic hepatitis virus by minimizing time and labor.;COPYRIGHT KIPO 2014