Fabrcation method of label free bio-imaging nanoparticles based on targeting agent conjugated polyacrylonitrile nanoparticles

摘要

PURPOSE: A manufacturing method of nano-particles for non-label bio-image using polyacrylonitrile nano-particles on which target-oriented molecules are attached is provided to manufacture polymer nano-particles which can be used for agent for bio-images without additional fluorophore addition. CONSTITUTION: A manufacturing method of nano-particles for non-label bio-image using polyacrylonitrile nano-particles on which target-oriented molecules are attached comprises the following steps: manufacturing polyacrylo nitrile nano-particles having photo luminescent character of 10-50 nano meters by microemulsion polymerization using surfactant under ultrasonic wave; introducing various functional groups to surfaces of the nano-particles through a vapor deposition method; fixing target directive molecule in the functional group of nano-particles; and applying the nano-particles to the bio-image agent for imaging targeted specific cell.