METHOD FOR QUANTITATIVE ANALYSIS OF CELL-DEATH PROCESS USING ABSORPTION-BASED IMAGE CYTOMETRY

摘要

The present invention relates to apoptosis quantitative analysis method using absorbance image analysis, and more specifically, to take a photograph of the formed image after the absorption dye treatment in a single cell layer induced apoptosis by exposure to a chemical sample and the absorbance of each cell quantitatively It relates to apoptosis quantitative analysis to be analyzed by. According to the present invention, by using multivariate analysis of cell-specific absorption and form factor, the process of apoptosis of individual cells in comparison with normal cells can be more accurately quantified compared to conventional absorption-based cell death assays such as MTT detection. Therefore, it can be used as a high efficiency, high density cell-based microfluidic chip, pre-in-vitro screening device and new chemical cytotoxicity evaluation device when developing new drugs.