METHOD USING A MICRO-PLATE FOR MEASURING THE CONCENTRATION OF TBARS, CAPABLE OF MEASURING THE CONCENTRATION OF THE TBARS EXISTED IN A BIOLOGICAL MATERIAL MASSIVELY AT HIGH SPEED

摘要

PURPOSE: A method using a micro-plate for measuring the concentration of TBARS(Thiobarbituric acid reactive substances) is provided to measure the concentration of TBARS in a biological material by using a regression equation based on a concentration value measured in the micro-plate by using fluorescence and absorbance.;CONSTITUTION: A method using a micro-plate for measuring the concentration of TBARS comprises: a step of generating the TBARS by adding TBA(Thiobarbituric Acid) to a biological material and reacting the same; a step of transferring a reactant including the TBA to the micro-plate; a step of measuring the concentration of the TBARS existed in the reactant on the micro-plate with a spectrophotometric method by using a micro-plate reader; and a step of estimating calculated value A as the concentration of the TBARS. Formula 1: A=1.830×B-0.685×C+0.0035×D-0.282; B,C,D are obtained by the prior step.;COPYRIGHT KIPO 2013