Apparatus for analyzing single protein-protein interactions in whole cell lysates

摘要

to the single molecule level, protein-protein interaction analysis apparatus is disclosed. The present invention, the first protein is a combination of supplying a first protein in a first biological molecule chain binding protein minute sample loading unit (substrate that itself is attached to the substrate is loaded is coupled to a first protein and a first protein binding molecular sieve the induction and, when the first protein and a first protein binding molecular sieve combined, the cell markers of the cell that contains the second solution is supplied to the substrate protein is provided), the surface of the substrate that is loaded in the sample loading unit In the state where the supply to the optical unit, and the cell solution, the substrate for generating a near-field having a first wavelength, the wavelength of the first to the second wavelength in the substrate surface according to a first protein interacting with a second protein and having a optical measurement to detect changes, the optical measurement unit, and measuring the fluorescence signal integration of the second wavelength for a predetermined time. According to the invention, the protein in the actual cell environment-protein interaction to be able to analyze single molecule level. In addition, according to the present invention, in the actual cell environment, even if the other protein is involved in specific protein-protein interactions for the influence it is possible to analyze ;