Process for chromosomal integration and DNA sequence replacement in Clostridia

摘要

The present invention relates to a new method for replacing or deletion in a DNA sequence in the high-efficiency and easy applicable, Claus tree Dia (Clostridia) to perform industrial-level. This method is useful to transform some of the loci in the Claus dia tree in a conventional manner. The method is based on the cloning vector carrying at least two marker genes. ; Claus Tree Boutique strains, chromosomal integration, DNA substitution, a marker gene, cloning vectors