CALCULATION METHOD OF BEERCONTAMINATING INSECTS INFLOW TIME USING MARKER GENE FROM THE INSECTS

摘要

PURPOSE: A calculating method of beer inflow timing of insects by using marker genes of insects is provided to enhance accuracy by drawing quantitative formula. CONSTITUTION: A calculating method of beer inflow timing of insects comprises the following steps: obtaining Ctc which is Ct value of marker gene of control group from the quantitative result of real-time PCR; performing quantitative real-time PCR for marker gene of the standard samples after specific duration is passed from the extinction day of the standard sample; obtaining Ct value of marker gene of the standard sample from the real-time PCR resu obtaining the Cts which is Ct value of the marker gene of the standard sample according to the elapsed time; calculating Ct ratio by using Cts from the second step and Ctc from the first step; and obtaining the standard curve by using the Ct ratio. The control group and the standard sample are allogenic.