Method for plantlet formation of blueberry cv. Toro, Legacy or Oregonblue using leaf explant culture

摘要

PURPOSE: A method for plantlet formation of blueberry cv. toro, legacy or oregonblue is provided to increase the number of plantlets during a growth culturing process since a multi-shoot formation rate is higher than a meristem-tip culture. CONSTITUTION: A blueberry leaf is taken into a medium(1) to induce an initial callus and multi-shoot. 1 to 3 mg/L of Zeatin Riboside, 30 to 60 mg/L of FeNa-EDTA, 50 to 150 mg/L of myo-inositol, 1 to 3 mg/L Thiamine HCl, 0.5 to 1.5 mg/L of Nicotinic acid, 0.5 to 1.5 mg/L of Pyridoxine HCl, 10 to 30 g/L of sucrose, and 4 to 8 g/L of agar are added to the medium and then cultured in a next medium(2) to form a plant body. In the next medium, Zeatin Riboside is added at 1/10 of the above concentration or not added, or the medium(2) is cultured in a medium added with 0.1 to 3 mg/L of IBA(Indole-3-Butyric Acid) to induce formation of rood by circulating from a culturing chamber to a greenhouse. The medium(1) includes 1 to 3 mg/L of zeatin, 30 to 60 mg/L of FeNa-EDTA, 50 to 150 mg/L of myo-inositol, 10 to 30 g/L of sucrose, and 4 to 8 g/L of agar. [Reference numerals] (AA) 14 weeks; (BB) 18 weeks; (CC) 24 weeks; (DD) 34 weeks; (EE) 3 months