COMPOSITIONS AND METHODS OF SUPPRESSING EXPRESSION OF FACTOR VII GENES

摘要

1. A double-stranded ribonucleic acid molecule capable of suppressing expression of the factor VII gene in vitro by at least 70%. 2. The double-stranded ribonucleic acid molecule according to claim 1, wherein said double-stranded ribonucleic acid molecule comprises a sense and antisense strand, and an antisense strand is at least partially complementary to the sense strand, the sense strand comprising a sequence that is at least 90% identical to the part of the mRNA encoding factor VII, wherein said sequence (i) is localized in the complementarity region of said sense chain to said antisense chain, and (ii) said sequence with Toit of at least 30 nucleotides in dlinu.3. A double-stranded ribonucleic acid molecule according to one of claims 1 or 2, comprising nucleotides 1-19 of the sequences SEQ ID No: 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425 , 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, and 438.4. The double-stranded ribonucleic acid molecule according to claim 3, wherein the antisense strand further comprises a protrusion of 1-5 nucleotides from the 3 ′ end, preferably 1-2 nucleotides in length. The double-stranded ribonucleic acid molecule according to claim 4, wherein the protrusion of the antisense strand includes uracil or nucleotides that are at least 90% complementary to the mRNA encoding factor VII.6. The double-stranded ribonucleic acid molecule according to claim 3, wherein the sense strand further comprises a protrusion of 1-5 nucleotides, preferably 1-2 1-2 nucleotides, from the 3'-end. The double-stranded ribonucleic acid molecule according to claim 3, wherein the protrusion of the sense strand includes uracil or nucleotides that are at least 90