A method for detecting M. tuberculosis genotype VEIJING IN REAL TIME

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: invention refers to medicine and microbiology. What is presented is a method for real-time detection of Beijing genotype mycobacterium tuberculosis by detecting IS6110 element insertion in a locus of dnaA-dnaNgenome differing by the fact that real-time PCR is carried out with the use of two specific primers5`-AGATCAGAGAGTCTCCGGACTCA and 5`-CGCCGGGACTGTATGAGTCT and fluorescence-labelled probe R6G-5`-TGTGCACAGCGACACTCACAGCCA-3`-BHQ2; the result is assessed by recording a fluorescence signal in R6G canal at wavelength 555nm; if a sample contains the DNA of the Beijing genotype mycobacterium tuberculosis strain, an exponential growth of the PCR fluorescence signal when a pure DNA analysed is observed between 10 to 15 cycles, and when cell lysates analysed - between 15 and 20 cycles.;EFFECT: invention can be used for laboratory detection of Beijing genotype mycobacterium tuberculosis.;3 dwg, 2 ex