POLYNUCLEOTIDE, WHICH CODES MUTANT RECOMBINANT IgA1 PROTEASE of Neisseria of meningitidis OF SULFUR PYRROLIDONE GROUP B, RECOMBINANT PLASMID DNA, CONTAINING a POLYNUCLEOTIDE, THE HOST CELL, WHICH CONTAINS PLASMID DNA, RECOMBINANT IgA1 OF THE PROSTHESIS of Neisseria of memingitidis OF SULFUR PYRROLIDONE GROUP B, METHOD OF OBTAINING RIPE FORM Of igA1 OF PROTEASE

摘要

FIELD: biotechnology.;SUBSTANCE: proposed enzymatically inactive IgA1 protease with replacement Ser267Ala for use as a component of a polyvalent vaccine designed to protect people against meningococcal infection and other microorganisms, which pathogenicity is caused by IgA1 protease. The invention includes a polynucleotide encoding the said mutant form of IgA1 protease of Neisseria meningitidis of serogroup B, comprising the said polynucleotide, a recombinant plasmid DNA, the strain Escherichia coli - producer of a mutant form of IgA1 protease according to the invention, the method of preparing a recombinant form of the enzyme using a technology of recombinant DNA, and recombinant inactivated IgA1 protease.;EFFECT: increased level of immunogenicity.;6 cl, 1 tbl, 5 dwg, 7 ex