METHOD genotyping polymorphism rs3825942 gene LIZILOKSIDAZOPODOBNOGO Enzyme 1 TYPE IN HUMANS

摘要

Rs3825942 polymorphism genotyping method liziloksidazopodobnogo enzyme gene in human type 1, wherein the polymorphism genotyping is carried out by polymerase chain reaction and restriction fragment length polymorphism using specially chosen primers SEQ ID NO 1-2 and differentiation FrioI endonuclease G and A alleles of the polymorphism rs3825942 LOXL1 gene wherein the wild-type homozygous genotype GG correspond to the restriction fragment length sizes of 120, 81 and 34 base pairs, homozygous mutant genotype IMPs AA - restriction fragment length sizes 154 and 81 nucleotide pair, heterozygous genotype GA - restriction fragment length sizes 154, 120, 81 and 34 base pairs.