METHOD OF OBTAINING THERAPEUTIC AGENT FOR PREVENTION OF DISEASE AND TREATMENT OF TICK-BORNE ENCEPHALITIS

摘要

1. A process for preparing a therapeutic agent for preventing and treating diseases borne encephalitis people where prepared aptamers tertiary structure having a steric matching and is capable of forming a stable complex with the tertiary structure of the surface protein of the virus, characterized in that as a component of a protein complex using a fragment of the surface E protein of tick-borne encephalitis virus, corresponding to amino acid residues 50-250 of the protein, the gene which is amplified and cloned into plasmid vector cloned recombinant protein is expressed, the recombinant protein was purified and immobilized on a plate, hereinafter from the initial oligonucleotide pool using 15 exponential enrichment cycles, SELEX, performed selection of specifically binding to the viral protein aptamers, wherein at each step sorption carried denaturation 25 pM single stranded oligonucleotide further denatured aptamer pool is applied to a well with an immobilized protein, incubated and washed free of unbound oligonucleotides associated protein aptamers are removed from the surface of the buffer with low ionic strength and high pH, ​​at the same aptamer pool after each step sorption amplified derived amplicons double-stranded aptamer library is purified and reacted asymmetric polymerase chain reaction (PCR) to produce single stranded product, which purified by sorption to obtain the title produkta.2. A method according to claim 1, characterized in that said protein gene fragment was amplified with primers E-ended 5'-GACTCCATCCATATGGAGAATCCTGCC-3 'and 5'-TACACGTCCTCGAGCACGGCGTG-3', then the floor