METHOD AND KIT FOR ENZYME-LINKED THROMBIN-BINDING ASSAY ON C1 INHIBITOR FUNCTIONAL ACTIVITY

摘要

FIELD: medicine.;SUBSTANCE: there are presented a method and a kit for enzyme-linked thrombin-binding assay on C1 inhibitor functional activity. The method implies thrombin sorption in microplate wells, introduction of test samples containing the functionally active C1 inhibitor to be analysed, incubation that involves C1 inhibitor binding to thrombin, and measurement of the C1 inhibitor-bound amount by an enzyme conjugate with antibodies and a substrate of said enzyme. The kit comprises a flat-bottomed microplate with the sorbed preparation of thrombin, the enzyme conjugate with the human C1 inhibitor antibodies, a substrate buffer and a reference for measuring the active C1 inhibitor.;EFFECT: group of inventions provides the novel method and kit for enzyme-linked thrombin-binding assay on C1 inhibitor functional activity.;2 cl, 2 dwg, 2 ex