METHOD FOR INCREASING ANTIGEN IMMUNOGENECITY OF B. pseudomallei ANTIGENS IN EXPERIMENTAL MELIOIDOSIS

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: method comprises immunising the animals with the use of surface melioidosis antigens in the liposomal form, and for the purpose of the additional stimulation of the cell immunity mechanisms, the immunomodulator Bestim is added to cytokines. The stimulant action of the preparations on the immune system is assessed in dynamics of the immune response as shown by the TDTH level and a degree of chemiluminescence response to zymosan of peritoneal macrophages in the immunised mice. The protective properties of the antigens are stated by mortality rates (the percentage of surviving animals and the average life expectancy) after the control infection with the virulent culture of B. pseudomallei. The immunogenic and protective properties of the antigens are substantially increased through the use of the antigen in the liposomal form and the addition of the cell immunity activator Bestim into the immunisation schedule.;EFFECT: method shall provide higher immunogenicity of the melioidosis antigens while developing the preparations for specific prevention of melioidosis infection.;6 tbl, 2 ex