METHOD TO PRODUCE POSITIVELY CHARGED PROTEIN FRACTIONS WITH INHIBITING ACTIVITY RELATIVE TO TRIPSIN IN GROWING POPULATION OF Escherichia coli

摘要

FIELD: biotechnologies.;SUBSTANCE: method includes conservation of cells in presence of buffered 80-90% glycerine with the following removal of cell shells with 3% triton X-100, extraction with growing concentrations of salts: 0.14 M, 0.35 M; 2 M NaCl, 6 M guanidine hydrochloride with 0.1% b-mercaptoethanol, extraction of positively charged proteins from above fractions with the help of ion-exchange chromatography with amberlite IRC-50 in the continuous gradient of guanidine hydrochloride: 6%, 8.9%, 10.6%, 13% in 0.1 M potassium-phosphate buffer pH 6.8 and detection of inhibiting activity in them relative to tripsin.;EFFECT: invention may be used to analyse molecular-genetic mechanisms of procaryote cell structure generation and role of protein components in their organisation, and also features of genome remodelling.;9 dwg, 1 ex