METHOD FOR CREATING BIOLOGICAL MODEL OF MODERATE GROWTH INHIBITION OF TUMOUR AND METASTASES IN LEWIS LUNG CARCINOMA WITH PROLONGED CYCLOPHOSPHANINE-INDUCED LEUKOPENIA IN MICE

摘要

FIELD: medicine.;SUBSTANCE: what is involved is simulating moderate growth inhibition of tumour and metastases in Lewis lung carcinoma with prolonged cyclophosphanine-induced leukopenia in mice by intraperitoneal introduction of cyclophosphane in 1/3 ADR - 3 times, 83.3 mg/kg each on 6th, 12th and 18th post-transplantation days. On the 3rd day after each introduction, peripheral blood is analysed (total leukocyte count, leukogram) - the lowest WBC count is determined. The clinical effectiveness is assessed at the end of the experiment on the 21st post-transplantation day as shown by tumour weight, a percentage of growth inhibition, a metastasis rate, the number of lung metastases, a metastasis inhibition index.;EFFECT: new biological model of carcinoma with comorbid prolonged leukopenia enables weighing the contribution of substances of different nature to the anti-cancer clinical effectiveness and to the elimination of white blood cell toxicity when the above substances are included into the therapy.;1 ex, 1 tbl