METHOD OF PRODUCTION OF R-BRUCELLAR ERYTHROCYTIC ANTIGEN FOR INDIRECT HEMAGGLUTINATION TEST (IHT)

摘要

FIELD: veterinary medicine.;SUBSTANCE: method of production of R-brucellar erythrocytic antigen for indirect hemagglutination test (IHA) includes production of formalinised red blood cells of sheep, their sensibilisation with sensitine obtained by growing bacterial mass of brucella, its washing-off with the hypertonic solution of sodium chloride, inactivation, extraction and separation of sensitine by centrifugation. At that the antigen is taken out from the strain B.abortus 16/4 by acting on the bacterial cells with 0.5% concentration of sodium dodecyl sulfate at the temperature of 68-70°C for 60 minutes.;EFFECT: invention enables to produce a diagnostic preparation of high performance for diagnostics of brucellosis in animals.;5 tbl, 1 ex