STRAIN OF ENTEROVIRUS COXSACKIE B6 SELECTIVELY INFECTING AND LYSING TUMOROUS HUMAN CELLS in vitro

摘要

FIELD: biotechnologies.;SUBSTANCE: described strain is produced by performance of a series of adaptation passages of a parent strain of the virus ZhEV-15 Coxsackie B6 on the culture of cells HEK293 highly sensitive to this virus and the neoplastic cell line C33A (HPV-negative carcinoma of human uterine neck), with production of a new strain ZhEV-15^L of the virus Coxsackie B6. The strain selectively infects and lyses tumorous human cells and has a fragment of a genome sequence represented in the dwg. 2, being its marker criterion. The strain is deposited in the State Collection of the Federal Service for Oversight of Consumer Protection and Welfare of agents of viral infections and rickettsial diseases of the Federal Budget Institution of Science State Science Centre of Virusology and Biotechnology "Vector" under the registration number V-576.;EFFECT: invention provides for the possibility of promising development of a viral oncolytic preparation on its basis for therapy of malignant human tumours and definition of a full nucleotide sequence of its genome.;3 dwg, 4 tbl, 2 ex