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Method and device for the quantitative measurement has a high rate of targets biomolecular presented on or in a support for biological analysis.

机译:用于定量测量的方法和装置具有高比率的生物分子靶标,该靶标存在于生物分析中或生物分析中。

摘要

The present invention relates to a device and a process for the quantitative measurement at a high rate of targets biomolecular to the surface or in the thickness of a support plane of analysis biologique.le process according to the invention comprises the following steps: a) is focused and superimposed on each measurement point of this support at least two laser beams (f) by simultaneous crossings of these beams, in order to extract a hot plasma confined (p) comprising a chemical element to quantify present in the targets and another chemical exogenous to the targets and present in a known quantity on this support, b) it detects and analysis for each measurement point, the luminous lines of transmission of each plasma, which correspond to the element to be quantified and to the exogenous element, by measuring the intensity of these lines, puisc) is determined via a prior calibration of the lines of the element to quantify establish a correlation between the intensities of the lines which are adapted to this element and the concentrations of the latter in the mixture; in proportions known to the element to be quantified and the exogenous element, here concentration in each measurement point of the element to quantify.
机译:技术领域本发明涉及一种用于以高速率定量分析生物体的表面或在支撑生物体的支持平面厚度内的靶标的定量测量的装置和方法。根据本发明的方法包括以下步骤:通过同时交叉至少两束激光束(f)将其聚焦并叠加在该支撑物的每个测量点上,以提取热等离子体(p),该热等离子体包含(p)包含化学元素以量化目标中存在的其他化学外源到目标并以已知的量存在于该载体上; b)通过测量样品的浓度,检测并分析每个测量点的每个等离子体的发光线,这些线对应于要定量的元素和外源元素。这些线的强度puisc)是通过事先对元素的线进行校准来确定的,以量化建立线的强度之间的相关性。 ich适用于该元素及其在混合物中的浓度;以待量化元素和外源元素已知的比例(此处为要量化元素的每个测量点中的浓度)表示。

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