Brief description of the study of the effect of at least one biocide on a strain of mold

摘要

The process comprises performing a first culture of a strain of fungus determined on a culture medium for a specified period (T), simultaneously performing a second culture of same strain of fungus on the same culture medium, during the same period, in the presence of determined quantity of a biocide, performing the photograph of an area of the same surface of the first and second cultures at the end of the culture period, calculating total combined surface area of spores and the total accumulated length of germinated mycelium spores from photograph. The process comprises performing a first culture of a strain of fungus determined on a culture medium for a specified period (T), simultaneously performing a second culture of same strain of fungus on the same culture medium, during the same period, in the presence of determined quantity of a biocide, performing the photograph of an area of the same surface of the first and second cultures at the end of the culture period, calculating total combined surface area of spores and the total accumulated length of germinated mycelium spores from photograph through analysis and computerized calculation units, assimilating the total spores at a single spore forming a disc of virtual radius (r) that calculates from the total combined surface area of spores, calculating a ratio (R) between the total length of mycelium and virtual radius for photography representative of an elongation rate for the first and second cultures, calculating the elongation percentage of the second culture with respect to the first culture from the ratio (R) obtained from each culture, and discriminating the spores and mycelium from the culture medium by calorimetric analysis of each pixel in the photographs according to a red-green blue (RGB) calorimetric system and assigning fixed scalar value to each pixel such that the scalar value is provided by a triple of RGB value in the calorimetric system. The photograph step is performed for 4-48 hours. The photographs have a resolution of 72 pixels by an image width unit, and are performed under light microscopy at a magnification of 500%. The first and second cultures are performed using a calibrated solution of spores from the strain of fungus. The concentration of the calibrated solution is selected so as to obtain 20-50 spores by photograph, and is 2.10 5spores by ml spread evenly over identical Petri dishes. The elongation percentage is compared with a determined threshold value for the biocide considered with the reference strains of the same species, which allow to classify the strain studied as mold-resistant or non-resistant to the considered biocide.