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MANUFACTURING METHOD OF SUGAR CHAIN CHIP FOR DETECTING TOXIN OR THE LIKE WITH HIGH SENSITIVITY BY MICROWAVE IRRADIATION

机译:微波辐照检测高灵敏度毒素或糖样的糖链芯片的制备方法

摘要

PROBLEM TO BE SOLVED: To provide a method that can fix a sugar chain to a sensor chip in a short time and can manufacture a sugar chain chip for detecting toxin or the like to be a detected object with high sensitivity.SOLUTION: In a manufacturing method of a sensor chip for detection of protein derived from bacteria or viruses, or organism toxin, a sugar chain-containing compound having a sugar chain specifically connected to the protein derived from bacteria or viruses, or the organism toxin is fixed on a substrate surface. In the method, a substrate is immersed in the solution of the sugar chain-containing compound and microwaves are radiated in a state of keeping constant temperature of 30°C to 45°C, and thereby the sugar chain-containing compound is fixed to the substrate surface.
机译:解决的问题:提供一种方法,该方法可以在短时间内将糖链固定到传感器芯片,并且可以高灵敏度地制造用于检测毒素等的糖链芯片,从而将其作为被检测物体。用于检测源自细菌或病毒的蛋白质或生物毒素的传感器芯片的方法,将具有糖链的糖链化合物特异性连接至源自细菌或病毒的蛋白质或生物毒素的方法固定在基质表面上。在该方法中,将基板浸没在含糖链化合物的溶液中,并在保持30℃至45℃的恒定温度的状态下辐射微波,从而将含糖链化合物固定在膜上。基材表面。

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