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Rice genes conferring broad disease resistance

机译:水稻基因赋予广泛的抗病性

摘要

A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out on Arabidopsis thaliana lines highly expressing rice full-length cDNA (rice-FOX Arabidopsis lines) which were prepared by using a FOX hunting system. As a result, one Arabidopsis thaliana line (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.
机译:在高表达水稻全长cDNA的拟南芥品系上进行了三步筛选,筛选对病原菌感染的抗性,筛选对病原性真菌感染的抗性和对水杨酸的敏感性。它们是使用FOX狩猎系统准备的。结果,成功选择了在所有三种类型的筛选中选择的一个拟南芥品系(品系K15424)。品系K15424携带水稻全长cDNA。产生了水稻过表达的AK070024,并使用T1代测定了对水稻细菌性叶枯病的抗性。结果,证实了AK070024-过表达的水稻对细菌性叶枯病和稻瘟病具有抗性。

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