Substrate probe, imaging method of detection method and the enzymatic activity of the enzyme activity by multiple nuclear magnetic resonance method

摘要

PROBLEM TO BE SOLVED: To provide a substrate probe capable of detecting enzyme activity with high accuracy and a method for detecting the enzyme activity by a multi-dimensional nuclear magnetic resonance method to use the probe.;SOLUTION: Multi-dimensional nuclear magnetic resonance measurement is performed by using a substrate probe, which is used for measuring enzyme activity by a multi-dimensional nuclear magnetic resonance method and characterized by containing an enzyme recognition site that is selectively recognized by an active-state enzyme, as at least one constitutional unit, a group to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, and the group is present specifically to the enzyme recognition site, thereby detecting the presence of the substrate probe and the enzyme activity. The enzyme activity is imaged by a multi-dimensional magnetic resonance imaging method.;COPYRIGHT: (C)2009,JPO&INPIT