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Acquisition of TR1 cells specific for food antigens or autoantigens from leukocyte or PBMC populations
Acquisition of TR1 cells specific for food antigens or autoantigens from leukocyte or PBMC populations
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机译:从白细胞或PBMC群体中获得对食物抗原或自身抗原特异的TR1细胞
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摘要
The invention relates to an in vitro method for the obtention of a food- or auto-antigen specific Trl cell population from a leukocyte or a PBMC population, which comprises stimulating the PBMC or leukocyte population with the food- or auto-antigen, and recovering the food- or auto-antigen specific Trl cell population from the stimulated cell population. Preferably, the PBMC or leukocyte population is re-stimulated at least once with the same antigen after step (1), in the presence of IL-2 and at least one interleukin selected from the group consisting of IL-4 and IL-13.The in vitro method may further comprise a third step of expanding the recovered antigen-specific Trl cell population, advantageously by contacting them with feeder cells capable of expressing factors necessary for said expansion. Preferably, the feeder cells are recombinant insect feeder cells.
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