adenoviral vectors VA-RNAs are not expressed

摘要

Ad vector that indicates the gene transfer efficiency equal to or higher than that of Ad vectors of conventional safety is high, ie, the present invention provides a Ad vectors can be used safely and effectively in the life science research and gene therapy. Furthermore, there is provided a vector producing Ad-amplifying cells can amplify the Ad vector. In the Ad vector genome, with Ad vectors VA-RNAs gene has been altered (VA-RNA II and / or VA-RNA I) VA-RNAs to prevent expression. In addition, Ad vector production, amplification cells, with cells transfected with the gene to express the VA-RNAs of Ad genome in cells constitutively expressing the E1 gene products to be used for the Ad vector amplification.