Adhesion to the culture aspect of the mannered null culture

摘要

PROBLEM TO BE SOLVED: To enable accurate measurement of a cellular thickness distribution, regardless of the changes in the refractive index of cells, accompanying the progress of cultivation.;SOLUTION: Two-dimensional distribution of phase information is obtained, in a state where a first culture solution L1 having a known first refractive index is stored so as to completely immerse cells S adhered onto a bottom face 1a of a culture vessel 1, by transmitting a light L of a predetermined wavelength through cells S and the first culture solution L1, and by photographing the transmitted light L. Next, two-dimensional distribution of phase information is obtained, in a state where a second culture solution L2 having a known second refractive index is stored to have a depth to completely immerse the cells S in the culture vessel 1, by transmitting the light L of the wavelength through cells S and the second culture solution L2, and by photographing the transmitted light L. Then, respective average values of the phase information are calculated. Next, the refractive index of the cell S is estimated, based on the ratio of the average value of the calculated phase information. Then, the thickness dimension is calculated by using the estimated refractive index.;COPYRIGHT: (C)2009,JPO&INPIT