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Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and their use in DNA sequencing
Photocleavable labeled nucleotides and nucleosides and labeled nucleotides and nucleosides and their use in DNA sequencing
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机译:可光裂解的标记核苷酸和核苷以及标记核苷酸和核苷及其在DNA测序中的用途
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摘要
Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3'-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. In another embodiment, the nucleotide or nucleoside with an unprotected 3'-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format.
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