Polarization monitoring of intracellular mitochondria

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting variation of a polarization state of mitochondria in a living cell.;SOLUTION: Variation of a surface plasmon resonance angle resulting from variation of a polarization state of mitochondria in a living cell is detected by using a surface plasmon resonance device. Otherwise, the variation of the surface plasmon resonance angle resulting from the variation of the polarization state of the mitochondria is detected by giving one or more substances to the living cell. A step of detecting the variation of the surface plasmon resonance angle resulting from the variation of the polarization state of the mitochondria is made into a step of detecting the variation in a time zone when the variation of the surface plasmon resonance angle results only from the variation of the polarization state of the mitochondria, preferably, a step of detecting the variation in a time zone after passage of 20 minutes, more preferably, a step of detecting the variation in a time zone after passage of 30 minutes, and further preferably, a step of detecting the variation in a time zone after passage of 35 minutes from the time when the substances are given.;COPYRIGHT: (C)2012,JPO&INPIT