How to analyze the collected cells were detected with the method of detecting a particular cell of a low concentration from heavily contaminating cell population

摘要

In the detection method of the CTC, there is no technique for counting the determined automatically in a short time state alive 1) CTC; 2) not realized the process of detecting counts the CTC that remains live, cultured and then harvested ; there is a problem measurable flow cytometer is not present and the total amount of sample 3) contamination-free. And a step of identifying counting CTC pretreatment process of concentrating the CTC, the fluorescent staining treatment, pre-treatment process, using the magnet by attaching the magnetic beads to the EpCAM expressing the CTC epithelial cell-derived wherein that concentrating the CTC Te, and fluorescently labeled by using the 5E11 antibody or EpCAM antibody surface markers of epithelial cells of the CTC, and performs nuclear staining two types of impermeable and cell membrane permeability, identification counting process is, using a flow cytometer liquid feeding system as a whole, including the flow cell can be replaced for each specimen and it is possible to sample liquid total volume measurement, automatically recognize the CTC by the ratio of the fluorescence signal strength of multiple, CTC and you are alive By counting by distinguishing the CTC is dead, there is provided a method for CTC detection comprises assessing the absolute concentration for the blood volume of death by CTC.